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ChIP assay - about input


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#1 panda11885

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Posted 07 November 2012 - 09:12 PM

Dear all,
I did chIp assay with 3 gene A, B, C. After Q-PCR, I did electrophoresis for input sample and target gene sample. the bands of all target gene samples are very clear. About input, the band of gene A and C are clear, but there is no band of gene B. I can't explain what happen. Please help me to improve my experiment. Thank you very much.

MAI

#2 pcrman

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Posted 08 November 2012 - 10:25 PM

I believe the problem could be due to technical issues: accidental loss of DNA, impurities in DNA, etc. Since you already got other samples to work, just repeat it to see if you are luck the next time.




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