Edited by LacquerHead, 05 November 2012 - 03:27 PM.
Submit your paper to J Biol Methods today!
1 reply to this topic
#1
LacquerHead
-
- Active Members
-
- 24 posts
member
4
Neutral
Neutral
Posted 05 November 2012 - 03:27 PM
Hi I am looking for a reliable way to extract histones (without disturbing their methylation state) from brain tissue? I am also getting nuclear fractions to investigate methylation differences by western blot but thinking that extracting the histones themselves from the nucleosome may yield cleaner western results. Any and all opinions on this are very much appreciated, thanks!!
#2
HOYAJM
-
- Active Members
-
- 142 posts
Veteran
9
Neutral
Neutral
Posted 06 November 2012 - 07:49 AM
Chromatin immunoprecipitation (ChIP) might be what you are looking for.
http://www.nature.co...ot.2008.66.html
http://www.nature.co...ot.2008.66.html
Also tagged with one or more of these keywords: Histone, Brain
Protocols and Techniques Forums →
Protein and Proteomics →
Histone extraction from S.cerevisiaeStarted by michael_p, 12 Jul 2013  histone, epigenetics, yeast
|
|
|
||
Protocols and Techniques Forums →
General Lab Techniques →
Histone precipitation and western blottingStarted by StefyDE, 28 Sep 2012 Western blot, histone
|
|
|
||
Protocols and Techniques Forums →
Protein and Proteomics →
Protein Isolation from mouse brain: problemsStarted by tribio, 18 Apr 2012 protein isolation, brain, mouse and 2 more...
|
|
|
||
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
RNA extraction from a 1mm brain tissue punch - uncertain about homogenisationStarted by Liinda, 29 Nov 2011 RNA, brain, hypothalamus, phenol
|
|
|
||
Protocols and Techniques Forums →
Immunology →
ELISA and Immunoassay →
ELISA Kits for proteins in brain homogenatesStarted by janap, 05 Oct 2011 ELISA, brain, proteins
|
|
|
