2 replies to this topic

[firefly2280]

Hi all

I could really do with some help. I am swabbing environmental sites, ie floors, surfaces (a 10cm3 area) and trying to extract DNA. Needless to say, Im not having a lot of luck, I think quantities are very low and inhibitors are present.

I have tried Qiagen colums with a bead-beating step first and also Phenol:chloroform. The column based method was better but neither give me a lot of DNA.

Does anyone have any ideas about increasing my yield please?

thanks

[El Crazy Xabi]

Lucky you have some...   I've been struggling with some samples that barely have any cells... and using 10 g of sample

If inhibitors are a problem you may want to try some MoBIO kits, they use a patented inhibitor removal technology (as they call it) that works pretty well with difficult samples.

Don't know if it would help:
http://www.mobio.com...dna-from-swabs/

And I think they send kit samples to test

Edited by El Crazy Xabi, 05 November 2012 - 07:55 PM.

[firefly2280]

Thanks

I tried the Mobio kits, maybe I should give them another go! Thanks for your help and good luck with your work...!