Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
* * * * * 1 votes

pET14b vector with insert transformed in BL21(DE3) RIPL cells

  • Please log in to reply
30 replies to this topic

#31 razasohail28@yahoo.com



  • Active Members
  • Pip
  • 9 posts

Posted 02 June 2013 - 11:07 PM

Hello Dear
Hoping you will be fine. i am using BL21 Codon Plus RP for expression of my protein using pGEX-KG Vector. Because pGEX-KG is Ampicillin Resistant so i use Ampicillin+Chloramphenicole Plates to culture the bacteria but when i transfer the colonies in Ampicillin resistant LB broth or the broth which contain Ampicillin+Chloramphenicole then the colonies not grow in broth. Colonies only grow in Cloramphnicole resistant broth. Then i have checked my plasmid using PCR but all colonies are negative (colony which grow on Chloramphnicole resistant broth). Please guide me in this issue

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.