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Where to obtain rat embryonic stem cells?


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#1 wdyeo

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Posted 04 November 2012 - 02:49 PM

Dear All,
May i know if there is any way i can obtain rat embryonic stem cells?
I would like to use the rat embryonic stem cells to be used as my positive control in my RT-PCR, to obtained the Oct4 band.
Thank you.

wdyeo

#2 Trof

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Posted 04 November 2012 - 03:17 PM

Usually rat embryo is a good option. Or buy a cell line.
But since you actually need only RNA and as a control, probably buying rat embryo RNA of certain age would be more affordable.
Like here, but that's just first search result, probably there are more and some supliers may even have RNA directly from rat ESC line.

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#3 wdyeo

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Posted 06 November 2012 - 03:24 AM

Dear Trof,
Thank you very much for the suggestion and link. Posted Image
But i hope my supervisor is willing to buy ~

wdyeo

#4 Trof

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Posted 06 November 2012 - 11:33 AM

Or you can go hunting into sewers ;)
(or pet shop and buy a pregnant rat, but that may have etical problems and also you would be required to have an animal handling certificate.. and also, really citing this kind of control RNA source would be.. pretty dificult)

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon





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