bacteriostatic assay... troubleshooting!!!
Posted 03 November 2012 - 02:14 AM
A monoclonal antibody generated against outer membrane proteins of my microbe has bacteriostatic activity. To evaluate this activity statistically, I am standardizing a bacteriostatic assay in microtitre plate (basically, recording the absorbance on hourly basis for 8 continuous hours). At early stage of standardization, things worked out very well. But I'm facing a wierd problem now. Positive control (organism in enrichment broth) which used to give a typical bacterial growth curve, is showing a drastic decrease in absorbance @ the first hour of incubation itself, and no change in absorbance in further hours. Tried everything to get rid of the problem - used fresh broth, tried different enrichment broths, fresh culture... no improvement. Not finding out the root of the problem. Kindly help.
Posted 03 November 2012 - 01:15 PM
Posted 04 November 2012 - 10:41 PM
I am using only fresh plates. I have changed the glassware to prepare the medium and buffers too. They did not help me.
Posted 06 November 2012 - 07:55 AM