I am venturing into a new field: stem cell and would like your help, please.
I isolated mouse mesenchymal stem cells (MSC) from compact bone, performed ficoll gradient centrifugation and cultured them in hypoxic conditions (5% oxygen). They have been in culture for about a week. Recently, I checked their morphology under the microscope and they seem weird to what is documented in literature. I have attached few photos. Which of these cells look like MSC?
Since cells are extracted from the compact, I am aware that there is more than one cell population isolated simultaneously. How do you proceed to obtain a homogenous MSC culture?
Please advise. Thank you!