BrdU immunofluorescence protocolBrdU
Posted 02 November 2012 - 05:31 AM
I am trying to study cell proliferation using BrdU immunofluorescence. I use control cells which I know are proliferating obtaining no results. I think it is because of BrdU concentration (I have read that I can only use 10microM). Have anybody try with higher concentrations?
Posted 05 November 2012 - 06:39 PM
Posted 06 November 2012 - 02:13 AM
Posted 06 November 2012 - 03:38 PM
Posted 14 November 2012 - 02:06 AM
I will try to incubate with BrdU overnight. Anybody have any extra advice?