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PBMC cells handling


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#1 claire84

claire84

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Posted 01 November 2012 - 10:51 AM

Hi guys,
maybe it's a silly question, but I'm not a cellular biologist and I need some help.
I isolated PBMC from the whole blood and then I seeded them in a 96 well plate. After incubation of 24h I would like to monitor the presence of a certain protein on their surface using a labelled antibody.

Do I need to treat them as adherent cells using trypsin before adding the mix with the antibody or can I resuspend them pipetting?
What do yo think?

thanks a lot!

#2 Curtis

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Posted 10 November 2012 - 12:59 AM

what is your desired method of detection? there is no need to use trypsin for suspension cells.




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