Isolation of IP complexes
Posted 27 November 2003 - 07:57 AM
I'm now using M2 Anti-Flag affinity gel(beads) from Sigma to pull down my IP complexes but don't know how to isolate those complexes from the beads without denaturing the proteins .(This beads bear a Flag antibody that recognize the fusion protein at N-terminal, Met-N-terminal and C-terminal).Normally,we use elution buffer like SDS sample buffer to elute the complexes before applying to a Western Blot.As far as I know SDS may denature the proteins and I really need the natural form to proceed to another assay,besides the usual WB.
I'd appreciate if anyone could give me an advice.Thanks.
Posted 15 December 2003 - 09:46 AM
Posted 23 February 2004 - 08:31 AM