Posted 31 October 2012 - 12:32 AM
My question is why do I tranform using DH5 alpha first instead of using SM10 λpir directly?
Please provide as much detail as possible, I'd really appreciate it.
Thank You very much
Posted 31 October 2012 - 01:24 PM
But it does not matter if you transform directly into SM 10 lambda pir
Posted 04 November 2012 - 01:46 PM
The genotype of the strain is KmR, thi-1, thr, leu, tonA, lacY, supE, recA::RP4-2-Tc::Mu, pir
What kind of plasmid do you have? More specifically: origin of replication? other characteristics?
Posted 31 January 2013 - 02:12 AM
I am using the vector pdsRed-express2; I am not sure about the origin of replication though...