TFE in inducing alpha-helix formation in proteins
Posted 30 October 2012 - 05:24 PM
I have been doing TFE titraion on polypeptides at 4 degrees celcius using CD, and noticed its varying effects on different polypeptides. It was effective in inducing alpha-helical structure formation in one polypeptide but not another. One of the peptide I tested was a 16mer and the other is a 32mer, the 16mer is a part of the 32mer. Does the length of the peptide influence the effect of TFE, since TFE showed its effect on the 32mer but not the 16mer (even at 80%TFE)? Any other possible reason for the difference in TFE effects on the two mers I tested?
Posted 01 November 2012 - 07:11 AM
Posted 01 November 2012 - 05:58 PM
Not even with the addition of TFE will it show secondary structure? I found that low temperature has some minor effect, but still more apparent as random coiling. Thanks for your help nonetheless!
Posted 02 November 2012 - 08:36 AM