3 replies to this topic

[Raquelina]

Hi everyone!

I've a question about ethanol precipitation of DNA.

Does anyone know how long can I leave the DNA precipitating at -80º?

Do you think a weekend is too much (well, it's too much, I normally performe this step in 20 minutes but...) ? I don't think that it will affect DNA integrity, but have no proof. If anyone has any idea it will help a lot!

Thanks in advance!

[phage434]

Indefinitely.  It's a good way to store DNA for really long times.  This is a common way of storing RNA to limit RNAse activity.

[bacchus]

about 2 hours  or longer

[Raquelina]

Thanks a lot! It really helps me out!