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Ethanol precipitation of DNA. How long...?

ethanol precipitation

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3 replies to this topic

#1 Raquelina

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Posted 30 October 2012 - 10:24 AM

Hi everyone!

I've a question about ethanol precipitation of DNA.

Does anyone know how long can I leave the DNA precipitating at -80º?

Do you think a weekend is too much (well, it's too much, I normally performe this step in 20 minutes but...) ? I don't think that it will affect DNA integrity, but have no proof. If anyone has any idea it will help a lot!

Thanks in advance!

#2 phage434

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Posted 30 October 2012 - 10:32 AM

Indefinitely. It's a good way to store DNA for really long times. This is a common way of storing RNA to limit RNAse activity.

#3 bacchus

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Posted 30 October 2012 - 07:49 PM

about 2 hours or longer

#4 Raquelina

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Posted 31 October 2012 - 02:54 AM

Thanks a lot! It really helps me out!




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