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Quantify Chromatin With Protein Assay?

ChIP Chromatin Quantification Chromatin Protein Assay

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#1 tm1987

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Posted 30 October 2012 - 09:01 AM

Hi all,

I'm currently working with ChIP in my lab and my PI wants me to figure out a way to quantify the amount of chromatin I get from each tissue sample (mouse brain). I know you can quantify using a DNA concentration assay, but my PI wants me to try out a protein assay because it should be easier(?) Anyone heard of this?

#2 chabraha

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Posted 01 November 2012 - 01:40 PM

Take an aliquot and reverse the crosslinks and purify the DNA..............either spec it using a nanodrop/picogreen assay or do qPCR next to a standard curve.
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