Hi all,
I'm currently working with ChIP in my lab and my PI wants me to figure out a way to quantify the amount of chromatin I get from each tissue sample (mouse brain). I know you can quantify using a DNA concentration assay, but my PI wants me to try out a protein assay because it should be easier(?) Anyone heard of this?
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Quantify Chromatin With Protein Assay?
Started by tm1987, Oct 30 2012 09:01 AM
ChIP Chromatin Quantification Chromatin Protein Assay
1 reply to this topic
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chabraha
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Posted 01 November 2012 - 01:40 PM
Take an aliquot and reverse the crosslinks and purify the DNA..............either spec it using a nanodrop/picogreen assay or do qPCR next to a standard curve.
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