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how to separate Antibody and Antigen in complexes

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Posted 30 October 2012 - 05:21 AM

I have a sample from immunoprecipitation column - polyclonal porcine antibodies in complexes with salmonella proteins. (all in PBS)
any suggestion how do I separate them and isolate only salmonella proteins?
Of course there exist many IP kits with protein A or G on beads or stuff, but I'm looking for some simple solution to do this without any kits.
thanks a lot for your replies

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