Jump to content

  • Log in with Facebook Log in with Twitter Log In with Google      Sign In   
  • Create Account

- - - - -

Help w/ Media Preparation


  • Please log in to reply
3 replies to this topic

#1 BRB

BRB

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 29 October 2012 - 06:15 PM

What volume (in mL) of each ingredient is needed to prepare 50mL of DMEM supplemented with 2% FBS and 2x antibiotics (100x stock)? Thank you in advance for your help!

#2 leelee

leelee

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 631 posts
49
Excellent

Posted 29 October 2012 - 08:17 PM

Firstly, this is really simple maths, so give it a go first and then we can set you straight if you are wrong.

And secondly, why do you want your antibiotics at 2x concentration?

#3 BRB

BRB

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 30 October 2012 - 03:20 AM

I believe it is 48ml DMEM + 1ml FBS + 1ml of 100x antibiotics.... is this correct? Thank you.

#4 leelee

leelee

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 631 posts
49
Excellent

Posted 30 October 2012 - 03:22 AM

Yup, see you didn't need our help after all :)




Home - About - Terms of Service - Privacy - Contact Us

©1999-2012 Protocol Online, All rights reserved.