What volume (in mL) of each ingredient is needed to prepare 50mL of DMEM supplemented with 2% FBS and 2x antibiotics (100x stock)? Thank you in advance for your help!
Help w/ Media Preparation
Started by BRB, Oct 29 2012 06:15 PM
3 replies to this topic
#1
Posted 29 October 2012 - 06:15 PM
#2
Posted 29 October 2012 - 08:17 PM
Firstly, this is really simple maths, so give it a go first and then we can set you straight if you are wrong.
And secondly, why do you want your antibiotics at 2x concentration?
And secondly, why do you want your antibiotics at 2x concentration?
#3
Posted 30 October 2012 - 03:20 AM
I believe it is 48ml DMEM + 1ml FBS + 1ml of 100x antibiotics.... is this correct? Thank you.
#4
Posted 30 October 2012 - 03:22 AM
Yup, see you didn't need our help after all













