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Help w/ Media Preparation


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#1 BRB

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Posted 29 October 2012 - 06:15 PM

What volume (in mL) of each ingredient is needed to prepare 50mL of DMEM supplemented with 2% FBS and 2x antibiotics (100x stock)? Thank you in advance for your help!

#2 leelee

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Posted 29 October 2012 - 08:17 PM

Firstly, this is really simple maths, so give it a go first and then we can set you straight if you are wrong.

And secondly, why do you want your antibiotics at 2x concentration?

#3 BRB

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Posted 30 October 2012 - 03:20 AM

I believe it is 48ml DMEM + 1ml FBS + 1ml of 100x antibiotics.... is this correct? Thank you.

#4 leelee

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Posted 30 October 2012 - 03:22 AM

Yup, see you didn't need our help after all :)




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