separate plant protoplast from bacteria prior DNA extraction
Posted 29 October 2012 - 07:33 AM
My work is focused on microbes-plant interaction using metagenomics. I have extracted DNA from Leaves and fruits and around 95% of the sequences i obtained were from the choroplast and mitochondria from the plant, and actually i am interested in the bacteria growing in this samples.
does someone know a protocol to separate the plant protoplast from the bacteria to use it before performing the DNA extraction?
Thank you so much for your help
by the way...congrats for the forum, it is really interesting!
Posted 29 October 2012 - 11:49 AM
Posted 10 November 2012 - 06:00 PM
Markx GH, et al. Dielectrophoretic characterization and separation of micro-organisms. Microbiology (1994), 140, 585-59.
Markx GH and Piethig R. Dielectrophoretic separation of cells: Continuous separation. Biotechnology and Bioengineering (1995) 45:4 337–343.
Edited by lab rat, 10 November 2012 - 06:02 PM.