I have the problem of immunoprecipitation. Please help me solve this problem.
I overexpressed the HA-tag expression vector in cells，and i can detect the exogenous HA tag protein by using western blotting.
But when I perform immunoprecipitation, I used HA tag antibody to pull down the protein complex of my HA-tag protein. I cant detect the HA-tag protein in the pulldown protein complex by using western blotting. My HA-tag protein has many zinc finger, i think whether the HA tag is embed in the protein structure, so HA tag antibody cant pull down this protein.
Please help me disolve this problem.
Thanks very much.
Edited by Curtis, 28 October 2012 - 10:11 PM.