Proteinase K digestion time.
Posted 27 October 2012 - 04:57 PM
Posted 27 October 2012 - 10:01 PM
Posted 27 October 2012 - 10:36 PM
Posted 27 October 2012 - 11:14 PM
Long time digestion may not interfere with DNA as all DNases are supposed to be inactivated, though as per http://www.piercenet...?fldID=06010216 they recommend short time of digestion. may be they have standardized time. Why you want to waste your time reinventing wheel?
I left some samples to digest overnight on Friday but am not able to work on them until Monday or Tuesday. Not sure if my colleague received my text about inactivating the enzyme and putting them in 4C.
There is potentially the issue of degradation of the DNA through acid hydrolysis, but this shouldn't be too much of a problem if you are using a buffered system.
Samples are in TNES buffer so I guess it isn't going to be a problem.
How stable is DNA at RT and at 55C while in TNES?
Posted 28 October 2012 - 12:38 AM