Cell count and seed, plz help...
Posted 27 October 2012 - 01:56 PM
Posted 27 October 2012 - 06:39 PM
It really is up to you to figure out if it is easier for you to think in terms of concentration of cells (that is, 3x10^5 cells/ml) or in total cell numbers (3x10^6).
You could use c1v1=c2v2 if you are happier to think of it in terms of concentration.
But either way you work it out, you should still come to the same volume of cell suspension. I think your confusion with the number of cells in 10ml is steering you wrong here.
You are right in that there will be 2x10^5 cells in 660ul of your cell suspension.
Don't know if that helps you
Posted 28 October 2012 - 11:25 AM
Posted 28 October 2012 - 12:49 PM
nope, it's as what leelee says (and it's much easier to use the formula)....or you can always try a little bit of logic or visualisation (for math atheists like myself) :
Thank you very much for reply, that 3x10^7 is a typo...considering that the number of cells are 3x10^6 in 10ml and we want 200,000...shouldn't we take 0.066ml (66ul)?
if you have 3,000,000 cells in 10 ml...then
you have 300,000 in 1 ml..
and 30,000 in 100 ul...... therefore
only about 20,000 in 66 ul
- hobglobin, personal comment about my beauteous photo......
Posted 30 October 2012 - 02:48 PM
I guess it is the same,
if u will calculate it in 10 ml it must give u thr same number as if u calculate it in 1 ml.
Math is the same here, if it is not the same, this means that u r making some mistake in calculation.
so for me it is easier to go for 1 ml calculation, better than adding extra zero then remove it again by division