PolyA tail essential?
Posted 27 October 2012 - 12:40 PM
I'm trying to over-express a protein in zebrafish and I have them in a vector that doesn't have a polyA signal. I will eventually clone it into a vector that has a signal, but I want to just see whether the expression of the protein is correct first. I was going to do an RNA run off and inject into the zebrafish. Will the lack of polyA signal affect the expression of protein?
Posted 27 October 2012 - 10:43 PM
Posted 29 October 2012 - 02:12 PM
Posted 08 November 2012 - 03:33 PM