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Acetone Precipitation of proteins


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#1 Izanloo

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Posted 27 October 2012 - 10:57 AM

Hi,
I have extracted protein from wheat grains by Tris-Acetic acid-2 Mercapto etanol. but the problem is that before Precipitation with Acetone, i added sample buffer for SDS-PAGE.
I am wondering whether i could do Acetone Precipitation while there is sample buffer or not?
please give me some advice.

many thanks

#2 Inbox

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Posted 27 October 2012 - 10:36 PM

Buffering action will decrease or neutralize the acetone. So depends to what extent it decrease or neutralize the acetone.




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