7 replies to this topic

[Sotirios]

Does anyone know which is the green-dye in HRP-Conjugate ready-to-use solution in ELISA kits?

[bob1]

Which company?

[Sotirios]

for example from abcam elisa kit

[bob1]

Probably TMB tetramethylbenzidine having looked at a product description on the Abcam website...

[Ben Lomond]

TMB is the chromogen that changes colour in the presence of substrate and HRP.

The green dye is simply there as a visual to aid in the loading of wells. You could do some detective work and match the absorption spectrum with common green dyes if your spec scans.

[bob1]

Ben Lomond, on 30 October 2012 - 06:42 PM, said:

The green dye is simply there as a visual to aid in the loading of wells.

TMB is blue/greenish, depending on pH, isn't it?

[Sotirios]

Indeed. The green dye is simply there as a visual to aid the loading of HRP-Conjugate solution. The green dye (probably food dye) must not affect HRP function.

[bob1]

Sotirios, on 31 October 2012 - 12:54 AM, said:

Indeed. The green dye is simply there as a visual to aid the loading of HRP-Conjugate solution.

Ah, I see what you mean - added before the substrate.  Nice idea.