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Green-dye in HRP-Conjugate solution


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#1 Sotirios

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Posted 26 October 2012 - 12:53 PM

Does anyone know which is the green-dye in HRP-Conjugate ready-to-use solution in ELISA kits?
Athanasiou Sotirios

#2 bob1

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Posted 27 October 2012 - 10:53 PM

Which company?

#3 Sotirios

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Posted 28 October 2012 - 01:23 AM

for example from abcam elisa kit
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#4 bob1

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Posted 28 October 2012 - 11:20 AM

Probably TMB tetramethylbenzidine having looked at a product description on the Abcam website...

#5 Ben Lomond

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Posted 30 October 2012 - 06:42 PM

TMB is the chromogen that changes colour in the presence of substrate and HRP.

The green dye is simply there as a visual to aid in the loading of wells. You could do some detective work and match the absorption spectrum with common green dyes if your spec scans.

#6 bob1

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Posted 30 October 2012 - 10:47 PM

The green dye is simply there as a visual to aid in the loading of wells.

TMB is blue/greenish, depending on pH, isn't it?

#7 Sotirios

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Posted 31 October 2012 - 12:54 AM

Indeed. The green dye is simply there as a visual to aid the loading of HRP-Conjugate solution. The green dye (probably food dye) must not affect HRP function.
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#8 bob1

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Posted 31 October 2012 - 11:35 AM

Indeed. The green dye is simply there as a visual to aid the loading of HRP-Conjugate solution.

Ah, I see what you mean - added before the substrate. Nice idea.




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