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problem with protein purification

Acetone Precipitation

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#1 Izanloo

Izanloo

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Posted 25 October 2012 - 12:27 PM

Hi,
I have extracted protein from wheat grains by Tris-Acetic acid-2 Mercapto etanol. but the problem is that before Precipitation with Acetone, i added sample buffer for SDS-PAGE.
I am wondering whether i could do Acetone Precipitation while there is sample buffer or not?
please give me some advice.

regards





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