ANyone know a good chemical/enzyme to use to dephosphorylate serines? I have used SAP treatment before to dephosphorylate tyrosines....is it efficient for serines too?
appreciate the help guys!
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John Forsberg
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Posted 25 October 2012 - 05:52 PM
I'm not sure about "all" serines, but we've used calf intestinal phosphatase to treat protein lysates to dephosphorylate serines on some target proteins. As near as I can tell, SAP and CIP are both alkaline phosphatases, and will work on most substrates with a phosphate.
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