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dephosphorylate serines

SAP

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#1 wizzkid

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Posted 25 October 2012 - 06:29 AM

ANyone know a good chemical/enzyme to use to dephosphorylate serines? I have used SAP treatment before to dephosphorylate tyrosines....is it efficient for serines too?

appreciate the help guys!

#2 John Forsberg

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Posted 25 October 2012 - 05:52 PM

I'm not sure about "all" serines, but we've used calf intestinal phosphatase to treat protein lysates to dephosphorylate serines on some target proteins. As near as I can tell, SAP and CIP are both alkaline phosphatases, and will work on most substrates with a phosphate.





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