Posted 24 November 2003 - 02:39 AM
Posted 19 January 2004 - 08:59 AM
What is your template, genomic DNA, plasmidic DNA?. It is obvious that your primers are not specific.
Have you checked homo and heterodimer formation?
Posted 20 January 2004 - 08:12 AM
Posted 22 January 2004 - 08:52 AM
Good luck..and DON'T GIVE UP!
Posted 23 January 2004 - 03:05 PM
Posted 08 March 2004 - 09:40 PM
you have quite a large segment to amplify, so what are your cycling times? Make sure you give your product enough time to be formed, you may have to go as much as 2mins for extension phase.