DNA Kit Stability
Posted 25 October 2012 - 03:18 AM
I have developed one DNA isolation kit for mycobacteria. this kit is based on Spin column principle.
Can anyone have an idea that how can i check the temperature based stability of my kit reagents. My kit contains buffers with salts and detergents, proteinase K and Lysozyme.
Posted 25 October 2012 - 12:26 PM
Posted 25 October 2012 - 09:50 PM
I had desided to keep my kit at 4°C, Room Temperature and at 37°C.
For Proteinase K & Lysozyme: Though we know that these enzyme will be in active state to some extent at RT and 37°C, is it necessary to keep these Enzyme at such temperature? can I put them with remaining buffers?
For Samples: What kind of sample should I use? My kit can be use to isolate DNA from both, Gram positive as well as Gram negative cultures. Should I use fresh cultures of E.Coli & B. subtillis?
Thank you in advance
Posted 25 October 2012 - 11:31 PM
You know more about your kit than i do - you are the expert on what sort of samples would be appropriate.