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HELP!! Anti-GFP antibody resuspended in 20% SDS!!!!

antibody antibodies SDS resuspend sodium dodecyl sulfate

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#1 SPombe3



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Posted 24 October 2012 - 10:32 AM


One of the students in our lab accidentally resuspended our 200ug Anti-GFP antibody in 500uL of 20% SDS solution instead of sterile water!! (The bottles were right next to each other)

What should I do from here??
As the lab manager, I am responsible for all of the reagents, and antibodies are way too expensive to just order more!
Since antibodies are proteins, is our stock completely ruined now??

I tried to think of some steps I could take from here:
1. Use them as is (will the antibodies even work now?)
2. Use lyophilization to dry out the samples in our vacuum/centrifuge. However, I'm afraid the SDS will precipitate with the antibody.

Any other ideas??

#2 bob1


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Posted 24 October 2012 - 12:31 PM

You could try dialysing the protein against a buffer without SDS.

#3 mdfenko


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Posted 25 October 2012 - 11:48 AM

you will need to add triton to the dialysis buffer if you want to have any chance of renaturing the antibodies.

i think they're shot. even if you succeed in regaining some function the results won't be reliable. you're going to have to bite the bullet and order more.
talent does what it can
genius does what it must
i do what i get paid to do

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