Cell numbers versus confluency
Posted 23 October 2012 - 08:13 AM
Which situation would you consider preferable? Equal confluency (ie, both treated at 80% confluency) or equal number of cells (ie, 1 million cells treated regardless of confluency)?
Posted 23 October 2012 - 09:25 AM
Another way would be to keep confluence the same and normalize for cell number at the end, i.e., express uptake in the form of umoles/million cells or something like that.
Posted 23 October 2012 - 05:59 PM
If you are worried that the state of confluence the cells have reached will have an effect on compound uptake, you could try examining uptake for each cell line at different densities to work out if this is going to be a problem.