I am a master student and I am doing my master thesis in the cloning of keratinase sequence in competent cell.I tried several different methods and procedure for the cloning and I got the colonies but when I did the mini prep I had the good concentration of the DNA but when run on agarosei could not see the band.. I have to stop my thesis at this point but I am unable to think What could went wrong for the failure of the cloning, I am really confused what reasons should I put it in my discussion and in the overview that what should be done in future to avoid this proble.. Any help and answer would be of great help to me... thanks and regards
molecular cloning failure
Posted 23 October 2012 - 05:18 AM
Posted 23 October 2012 - 06:35 AM
Posted 23 October 2012 - 10:02 AM
I have sent you a personal mail..kindly reply to it. thanks
We can't possibly help with so little information about what you are trying to do or what you have tried.