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Selenite F broth Salmonella color


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#1 prabhubct

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Posted 23 October 2012 - 04:31 AM

Why salmonella give pink color to Selenite F Broth culture?
“Those who mind don't matter, and those who matter don't mind.”
--  Bernard M. Baruch

#2 Phil Geis

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Posted 23 October 2012 - 06:15 PM

You can have a little red orange precipitate - not sure if it's Selenium oxide or sulfide - but probably not from any Salmonella.

#3 prabhubct

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Posted 23 October 2012 - 08:36 PM

Thanks for your reply. Does this precipitate could be expected in E.coli growing in Selenite F Broth after 24 hours.
If we subculture many times in selenite broth will that it be like selective media like Bismuth Sulfite agar?
“Those who mind don't matter, and those who matter don't mind.”
--  Bernard M. Baruch

#4 Phil Geis

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Posted 24 October 2012 - 03:13 AM

Don't think there's anything special about a bacterium in re. the precipitate.  You shouldn't see much growth of E. coli as the purpose of the medium is to enrich Samonella spp. (esp. S. typhi ) in the presence of coliforms.  Color might even be associated with the medium ageing.  Selenite medium is indeed selective




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