Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Selenite F broth Salmonella color


  • Please log in to reply
3 replies to this topic

#1 Inbox

Inbox

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 331 posts
21
Excellent

Posted 23 October 2012 - 04:31 AM

Why salmonella give pink color to Selenite F Broth culture?

#2 Phil Geis

Phil Geis

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 219 posts
15
Good

Posted 23 October 2012 - 06:15 PM

You can have a little red orange precipitate - not sure if it's Selenium oxide or sulfide - but probably not from any Salmonella.

#3 Inbox

Inbox

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 331 posts
21
Excellent

Posted 23 October 2012 - 08:36 PM

Thanks for your reply. Does this precipitate could be expected in E.coli growing in Selenite F Broth after 24 hours.
If we subculture many times in selenite broth will that it be like selective media like Bismuth Sulfite agar?

#4 Phil Geis

Phil Geis

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 219 posts
15
Good

Posted 24 October 2012 - 03:13 AM

Don't think there's anything special about a bacterium in re. the precipitate. You shouldn't see much growth of E. coli as the purpose of the medium is to enrich Samonella spp. (esp. S. typhi ) in the presence of coliforms. Color might even be associated with the medium ageing. Selenite medium is indeed selective




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.