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RNeasy kit + DNAse digest from Promega


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#1 Podge

Podge

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Posted 23 October 2012 - 03:13 AM

Hey,

I will extra some RNA using the RNeasy Lipid tissue mini kit. In the protocol they suggest an optional on-column DNA digest. I don't have the Qiagen stuff for the digest, but I have Promega RQ1 DNase.

My question now is if I can use the RQ1 for the on-column digest or if I should digest after the RNA extraction or even before (read this somewhere)?

Has anybody tried this?

Thanks!




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