Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Extracting Protein Using TriZOL


  • Please log in to reply
3 replies to this topic

#1 medschoolchat

medschoolchat

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 22 November 2003 - 01:56 PM

I have to extract my RNA and was wondering to save the supernatant for use to purify my protein from the same sample.

My question is, is this a acceptable protocol of protein extraction and if so, is there any problems that I have to be aware of when doing this.

Thanks in advance.

#2 medschoolchat

medschoolchat

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 24 November 2003 - 02:04 AM

I guess not too many people out here are using this forum...


what a shame! great idea though!

#3 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,165 posts
67
Excellent

Posted 24 November 2003 - 02:19 AM

It's possible but if you are serious about your protein work, you should start with standard protocol of isolating protein.

I have used trizol to extract DNA after RNA extraction, I never got desired results (low yeild) and it's tedious too.

hope it's useful.

#4 ros

ros

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 38 posts
0
Neutral

Posted 09 December 2003 - 04:29 PM

i've also had many probelms with trizol. both for RNA and protein isolations. i've found that for low RNA yeilds trizol is extremely ineffective. also for protein extractions, the protocol is extremely tedious and i have had problems with resuspending the proteins after isolation. i would also recommend that you use another proteion extraction method (many to choose from). but if you can get it to work for you then i dont see why it would not be an 'accepted' protocol.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.