Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

TCA IgG precipitation

TCA IgG purification

  • Please log in to reply
2 replies to this topic

#1 pablo.barra

pablo.barra

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 22 October 2012 - 07:24 AM

Hi there,
I want to precipitate antibodies with TCA. There is some way to recuperate the activity, or "renature" the protein?

(If u ask... i'm crosslinking Ab with some fluorescent particles, so i don't need to "purificate" the IgG, just precipite it, and store it. If u have any other idea, please tell me)

I would appreciate very much if u can help me with that.

(sorry for my bad english... i actually speak spanish, so i hope u can understand what i'm asking for)

Thank u guys.

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,803 posts
133
Excellent

Posted 22 October 2012 - 07:36 AM

why don't you use ammonium sulfate or acetone to precipitate the antibody?
talent does what it can
genius does what it must
i do what i get paid to do

#3 pablo.barra

pablo.barra

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 23 October 2012 - 05:39 AM

I will try. Thank u




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.