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RNA extraction from B cells attached to Dynabeads

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#1 chonela



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Posted 22 October 2012 - 04:20 AM

Hi, I am new in B cell separation. I am using CD19+ Dynabeads after Ficoll centrifugation. Does anybody have extracted RNA directly from the B cells attached to Dynabeads. When I use Detachabeads I think I lose material with the washing up. Any help will be appreciated.


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