Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

suitable DNA extraction and sample freezing for MSP


  • Please log in to reply
1 reply to this topic

#1 nazanin kia

nazanin kia

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 22 October 2012 - 02:32 AM

Hi every one!

I'm working on my thesis topic and I need to know some initial info about methylation analysis.
I was wondering if DNA extraction without commercial kits (eg. with laundry detergents) affects methylation status of the promoter?
and, does freezing my serum samples ( which I intend to get my DNA from it) changes the methylation pattern?

I'll really appreciate any recommendation and clues.

#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,165 posts
68
Excellent

Posted 01 November 2012 - 09:19 PM

The answer to either of your question is NO. Freezing DNA won't change DNA methylation status. Commonly used DNA isolation methods do not affect it either. But if you are going to use the DNA to do bisulfite conversion for analyzing methylation, you do need pure DNA.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.