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quantifying the activity of a transcription factor


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#1 mihoz

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Posted 21 October 2012 - 09:48 PM

I am working with a transcription factor & i need to identify its activity among different time points. is there a method which can do that?

#2 bob1

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Posted 21 October 2012 - 10:40 PM

You probably need some sort of reporter assay based on a known downstream target. This is usually done with luciferase assays.

#3 mihoz

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Posted 22 October 2012 - 05:01 AM

i am sorry i needed to be more specific... i need an in vitro test not an in vivo one.

#4 bob1

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Posted 22 October 2012 - 12:23 PM

Try in vitro transcription with rabbit reticulocyte lysate.




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