Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

PhiX174 DNA

  • Please log in to reply
1 reply to this topic

#1 biovinz



  • Active Members
  • Pip
  • 13 posts

Posted 21 October 2012 - 04:44 AM

Hi I have one doubt in Bacteriophage PhiX174 .. I will transform PhiX174 Dna into E. coli cells. after transformation we can isolate colonies for use any antibiotic resistance or antibiotic free medium?
Thank u in advance.

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,508 posts

Posted 21 October 2012 - 10:46 PM

PhiX174 won't have native antibiotic resistance so presumably you are cloning some of it into a plasmid - how would you then select for the plasmid?

Note: I think this is a homework question, so I'm making you think a bit...

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.