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Kinase pulldown

pulldown kinase substrate ATP-dependent interactio

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#1 ssaputo

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Posted 19 October 2012 - 06:00 AM

I am trying to do a pulldown assay. I have a His-tagged kinase and am trying to determine if it binds other epitope tagged proteins in an in vitro experiment. My question is: is the interaction between a kinase and its substrate ATP-dependent?

#2 bob1

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Posted 19 October 2012 - 06:16 PM

Depends on the kinase...





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