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Dilution help for Bradfords Assay


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#1 moabubaker

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Posted 19 October 2012 - 04:11 AM

Can someone help me understand why a sample has a final concentration of 1500ug/ml of BSA. If I add 2mg/ml of stock, 10ul of water and 150ul of bradfords reagent.

If someone can point me in the right direction of how they worked it out. Thanks a lot.

#2 mdfenko

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Posted 19 October 2012 - 08:22 AM

what volume of 2mg/ml did you use? by adding water you are diluting it so that you get a reading that is the equivalent of 1500ug/ml (1.5mg/ml) bsa (also, there are different responses with different proteins).
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