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[Question] primer sequence for zebrafish U6 in SYBR-qPCR for miR normalization?

miR zebrafish qPCR U6

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#1 lydialing

lydialing

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Posted 19 October 2012 - 01:33 AM

Dear all,
I’m trying to quantitate changes in some microRNAs in Zebrafish using the Ncode kit (Invitrogen, for RT) followed by SYBR-qPCR and I need an internal control. Most papers use U6, but the primer sequence is not mentioned, or worse, the primers seem not to amplify U6 but to other genes. For example, in this work, http://www.sciencedi...041008X12003493 , in table 1, the primer set
U6
For
60
TCGCTACGGTGGCACATA

Rev

TATGGAGCGCTTCACGG
actually targets to the gene >NM_201213.1 Danio rerio glutaminyl-tRNA synthetase (qars)
I don't think zebrafish U6 and qars are the same gene, or am I getting something wrong?

Do you know any primers for zebrafish U6 that works in SYBR-qPCR?
Or other choices to serve as an internal control for normalization?
Thank you in advance.





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