Making a Phosphate Buffered Saline
Posted 19 October 2012 - 12:26 AM
0.1M Sodium Phosphate, 0.15M NaCl.
I know this is probably pretty basic, but I was hoping someone could help me out with this.
Posted 21 October 2012 - 12:01 AM
I used Henderson-Hasselbalch equation for the sodium phosphate concentrations, using a pKa value of 6.865 (I'm a little confused on this as some places say it's 6.86 and some say it's 7.21)
7.2 = 6.865 + log (Disodium phosphate Dodecahydrate/Monosodium phosphate monohydrate)
24.490 grams of Disodium phosphate Dodecahydrate
4.363 grams of Monosodium phosphate monohydrate
For the NaCl I calculated:
8.766 grams of NaCl
Edited by Gradstudent78, 21 October 2012 - 04:18 AM.
Posted 23 October 2012 - 06:34 PM