Hi friends,
I am working on epigenetics(specifically Dna associated proteins). I have parrafinized tissue slides, please suggest me how can i make use of those slides in my work. simple histology has alraedy been done on those slides
Regards
Binsan
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suggestions for making use of tissue slides
Started by pakbiochemist, Oct 18 2012 12:19 PM
3 replies to this topic
#2
bob1
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Thelymitra pulchella
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Posted 18 October 2012 - 01:50 PM
What sort of histology - many of the dying processes are quite damaging to DNA...
#3
pakbiochemist
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Posted 18 October 2012 - 09:04 PM
we have done Haematoxylin Eosin (H&E) Staining, McMANNUS' PERIODIC ACID SCHIFF'S (PAS) Staining, & Masson’s Trichrome Staining but that is in another study not included in epigenetics.
i wanted to know how can i use these slides for epigenetic studies?
Regards
Binsan
i wanted to know how can i use these slides for epigenetic studies?
Regards
Binsan
#4
bob1
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Thelymitra pulchella
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Posted 19 October 2012 - 06:18 PM
Acid will degrade DNA quite quickly so I doubt they will be any use. No harm in trying though.
