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suggestions for making use of tissue slides


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#1 pakbiochemist

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Posted 18 October 2012 - 12:19 PM

Hi friends,

I am working on epigenetics(specifically Dna associated proteins). I have parrafinized tissue slides, please suggest me how can i make use of those slides in my work. simple histology has alraedy been done on those slides

Regards
Binsan

#2 bob1

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Posted 18 October 2012 - 01:50 PM

What sort of histology - many of the dying processes are quite damaging to DNA...

#3 pakbiochemist

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Posted 18 October 2012 - 09:04 PM

we have done Haematoxylin Eosin (H&E) Staining, McMANNUS' PERIODIC ACID SCHIFF'S (PAS) Staining, & Masson’s Trichrome Staining but that is in another study not included in epigenetics.
i wanted to know how can i use these slides for epigenetic studies?

Regards
Binsan

#4 bob1

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Posted 19 October 2012 - 06:18 PM

Acid will degrade DNA quite quickly so I doubt they will be any use. No harm in trying though.




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