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How do they make passage limited cells? - Tissue and Cell Culture - BioForum

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How do they make passage limited cells?


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#1 zodiac1505

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Posted 18 October 2012 - 09:26 AM

Does anyone know what technique companies use to make passage limited cells? I would like to order an engineered cell line and they have kits with passage limited cells (couple of divisions) included. I was wondering how they are able to do that.

#2 drwho

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Posted 18 October 2012 - 10:27 AM

one way is to irradiate the cells, which stops cell division. We have had such cells from perkin elmer eg http://www.perkinelm...12F_M1F-A12.pdf




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