i am preparing lymphocytes for c - culture with bacteria to examine effect of t cell on bacteria but . my cell viability is so low 60 %
1- passing head kidney of fish through stainless steel mesh in HBSS
2- centrifugation is 1600 RPm 4C for 5 minutes
3- percol separation
4- MACS separation
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low cell viability after percollow cell viability
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