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low cell viability after percol

low cell viability

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#1 haitham20eg

haitham20eg

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Posted 18 October 2012 - 08:24 AM

i am preparing lymphocytes for c - culture with bacteria to examine effect of t cell on bacteria but . my cell viability is so low 60 %

procedure :

1- passing head kidney of fish through stainless steel mesh in HBSS
2- centrifugation is 1600 RPm 4C for 5 minutes
3- percol separation
4- MACS separation




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