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a quastion on migration assay

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#1 Hala Al-Agha

Hala Al-Agha


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Posted 18 October 2012 - 03:03 AM

Hi everyone :)

I'm performing a migration assay on HCT116 cells, some call it "wound assay"
anyhow, everything is going well with the scraching technique and so.
but when i examine the cells under miscroscope I see lots and lots of floating cells over the scrach and almost all the well.
(picture attached)
Its like the media is not clean enough, hince: I wash with PBS first then add fresh medium!!!
Its been annoying, and im afraid it might affect my results.
Im new to the cultuing techniques and if what I metioned has to do with technical things please let me know

thanx :)

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