Western blot membrane imaging
Posted 17 October 2012 - 08:32 PM
Posted 18 October 2012 - 12:05 AM
Film is still the standard for these sorts of things, most of the cheaper camera based systems, such as the Biorad ones are terrible for taking images of chemiuminescent blots.
Posted 18 October 2012 - 06:48 AM
Do you mean I can even take a picture of it with my phone camera in shiny room if I develop using BCIP and NBT?
it depends how you develop your blot. for example if you use AP-conjugated antibody and develop using BCIP and NBT then you can use a normal camera, but if you develop with ECL solution (which is very popular) you may need chemiluminescent gel doc or system. I used to develop with X-ray method, but I don't recommend it, because the reagent you use stain everywhere.
I've using Kodak imaging station in another lab, but current lab doesn't have that.
We have Polaroid mp-4 land camera and ultra-violet light. Some one in our building must used this before because I found biomax film. But nobody knows who used that.
So I'm wondering if this camera would work with chemiluminescent.
Any advise is appreciated.
Posted 18 October 2012 - 09:12 AM
Posted 18 October 2012 - 10:00 AM
Is this correct?
Posted 18 October 2012 - 02:27 PM