the male and female templates are old whereas other unknown templates were new.
It was stored at 4 degrees because i use it everyday.
0
Multiplex PCR
Started by Mad researcher, Oct 17 2012 07:07 AM
multiplex PCR amplification sex determination
33 replies to this topic
#31
Mad researcher
-
- Active Members
-
- 85 posts
Researcher in making
6
Neutral
Neutral
Posted 01 November 2012 - 07:45 AM
Cheers,
Mad Researcher
Mad Researcher
#32
hobglobin
-
- Active Members
-
- 5,249 posts
Growing old is mandatory, growing up is optional...
60
Excellent
Excellent
Posted 01 November 2012 - 08:23 AM
To be sure I'd check the DNA with a gel.
And if you replaced all reagents without success, perhaps the thermal cycler is not working properly, or someone changed the program (accidentally hopefully)?
And if you replaced all reagents without success, perhaps the thermal cycler is not working properly, or someone changed the program (accidentally hopefully)?
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
#33
Mad researcher
-
- Active Members
-
- 85 posts
Researcher in making
6
Neutral
Neutral
Posted 01 November 2012 - 08:25 AM
i checked the DNA with the nanodrop though and it was fine, but yes i would check the DNA with the gel also.
As for the thermal cycler program change - i double check it to make sure its the right program.
As for the thermal cycler program change - i double check it to make sure its the right program.
Cheers,
Mad Researcher
Mad Researcher
#34
Mad researcher
-
- Active Members
-
- 85 posts
Researcher in making
6
Neutral
Neutral
Posted 03 November 2012 - 08:30 AM
hobglobin, on 01 November 2012 - 08:23 AM, said:
To be sure I'd check the DNA with a gel.
And if you replaced all reagents without success, perhaps the thermal cycler is not working properly, or someone changed the program (accidentally hopefully)?
And if you replaced all reagents without success, perhaps the thermal cycler is not working properly, or someone changed the program (accidentally hopefully)?
I checked the DNA with gel, they are fine.
Cheers,
Mad Researcher
Mad Researcher
Also tagged with one or more of these keywords: multiplex PCR, amplification, sex determination
Protocols and Techniques Forums →
Molecular Biology →
T7 on single stranded promoter sequence?Started by Guest_hbn_* , 10 May 2012  T7, DNA polymerase, amplification
|
|
|
||
Protocols and Techniques Forums →
ChIP and Next Generation Sequencing →
Small cell number ChIP-seq protocols - has anyone tried them?Started by Guest_angelawu_* , 03 Apr 2012 ChIP-seq, Protocol, WGA and 1 more...
|
|
|
||
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
|
|
|
