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Multiplex PCR

multiplex PCR amplification sex determination

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33 replies to this topic

#31 Mad Researcher

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Posted 01 November 2012 - 07:45 AM

the male and female templates are old whereas other unknown templates were new.
It was stored at 4 degrees because i use it everyday.
Cheers,

Mad Researcher

#32 hobglobin

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Posted 01 November 2012 - 08:23 AM

To be sure I'd check the DNA with a gel.
And if you replaced all reagents without success, perhaps the thermal cycler is not working properly, or someone changed the program (accidentally hopefully)?

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#33 Mad Researcher

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Posted 01 November 2012 - 08:25 AM

i checked the DNA with the nanodrop though and it was fine, but yes i would check the DNA with the gel also.
As for the thermal cycler program change - i double check it to make sure its the right program.
Cheers,

Mad Researcher

#34 Mad Researcher

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Posted 03 November 2012 - 08:30 AM

To be sure I'd check the DNA with a gel.
And if you replaced all reagents without success, perhaps the thermal cycler is not working properly, or someone changed the program (accidentally hopefully)?


I checked the DNA with gel, they are fine.
Cheers,

Mad Researcher





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